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CatalogQ89072; or equivalent), grab and remove brush samples from each tube, while pressing the brush against inside of tube to void any residual fluid. Wipe tweezers with germicidal disposable cloths, and air dry prior to handling next brash sample. Centrifuge extract for 5 minutes at 3000 rpm (room temperature; Allegra X-12R, Beckman- Coulter, Fullerton, California, USA; or equivalent). Transfer extracts to Deep Well Plates: a) Uncap each tube of extract.

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