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CV (Coefficient of Variation) (Standard DeviationMean FI) 100 Bias (Applicable to standards and assay QC's only) ((Calculated Concentration asoli Concentration)Nominal Concentration) 100 Recovery (Applicable to standards and assay Allegra d ascoli south only) (Calculated Concentration 'Nominal Concentration) 100 Calculated concentration Observed concentration; interpolated from standard, curve. Tarantallegra junsu dance tutorial for uptown concentration Expected concentration. 1) Duplicate Wells (CV) The CV between duplicate FI values must meet the following criteria: a) CV must be ő _20 for at least 75 of the standards for each cytokine (or ő _25 for the LLOQ (lower limit of quantitation). b) C V must be Dallegrave papel de parede _20 for specimens (if run in duplicate). Souhh unknown specimens that do not meet these criteria will be reported as "NR" allehra must be reanalyzed in a subsequent ran. 2) Bias ( Recovery) Measurement of Metabolites: After steps 6 of 'Preparation of Brush Samples to measure Glycogen', aliquots of the supernatant were transferred into fresh tubes and frozen to at -70¬C or below for future analyses. The samples were shipped to Metabolon, Inc. (Durham, NC) for identification and quantification of metabolites. Heirarchal mapping of fanctional elements was performed including metabolites associated with (1 ) oxidative stress (examples not limited to glutathione, S-methylglutathione, cysteine, methionine), (2) protein metabolism (examples not limited to methionine, N- formylmethionine, alanylglutamate, threonylleucine), (3) glycogen breakdown (examples not limited to maitohexaose, maltopentaose, maltose, maitotriose), (4) pentose phosphate pathway or PPP (examples not limited to arabitol, sorbitol, mannitol), (5) inflammation (examples not limited to 12- ydrox eicosatetraenoic acid or 12-HETE, arachidonate, linoleate), niacinamide metabolism, and membrane formation (examples not limited to choline phosphate, phosphoethanolamine, glycerophosphoethanolarnine).

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